Labelling of cock spermatozoa with radio-active phosphate

Studies on acrosome labelling of mammalian spermatozoa by radioactive sugars.

The localization of glycoprotein synthesis and storage was studied during acrosome formation in guinea-pig using fine-structure autoradiography after (3H)-fucose incorporation. Three days after (3H)-fucose injection, labelling in spermatids was concentrated in the matrix of developing acrosomes, and it was evident that the fucosylation of acrosomal glycoproteins largely overshadowed the fucosyl...

Active Learning for Dialogue Act Labelling

Active learning is a useful technique that allows for a considerably reduction of the amount of data we need to manually label in order to reach a good performance of a statistical model. In order to apply active learning to a particular task we need to previously de ne an e ective selection criteria, that picks out the most informative samples at each iteration of active learning process. This...

The Radio Active Ash

Sperm glyceraldehyde 3-phosphate dehydrogenase gene expression in asthenozoospermic spermatozoa

It has been suggested that the energy required for sperm motility is produced by oxidative phosphorylation while glycolysis seems to be an important source for ATP transmission along the flagellum. Some studies have investigated the chemical and kinetic properties of the enzyme glyceraldehyde 3-phosphate dehydrogenase to identify any changes in the regulation of glycolysis and sperm motility. I...

Chemical labelling of active serum thioester proteins for quantification

The complement serum proteins C3 and C4 and the protease inhibitor α-2 macroglobulin are all members of the C3/α-2M thioester protein family, an evolutionarily ancient and conserved family that contains an intrachain thioester bond. The chemistry of the thioester bond is a key to the function of the thioester proteins. All these proteins function by covalently linking to their target by acyl tr...